It depends on how you back them up. If you are backing up entire ISO images of the disc and using Daemon-Tools to mount the image as a virtual drive - TheaterTek can play them. Otherwise I dont know, I havent bothered to back up via any of the other methods.
I use smartripper to rip the VOB's and use PowerDVD to play back the VOB's. You get a minor pause when the player switches between VOB's. Works fine for me although sometimes I loose the audio stream when I try to use FF or REW.
If you have ripped the files to the hard drive you can play the contents of the VIDEO_TS directory in WinDVD. In fact just doubleclicking the .ifo file will launch windvd and play the film. No ISO files or Daemon tools or virtual drives required.
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